Rapid, sensitive structure analysis of oligosaccharides
نویسندگان
چکیده
منابع مشابه
Rapid, sensitive structure analysis of oligosaccharides (carbohydrateymass spectrometryystructure analysisysequencing)
We have developed an efficient method for the derivatization of oligosaccharides, wherein the oligosaccharide is efficiently ligated to a basic aminooxyacetyl peptide by oxime formation. The resulting glycopeptide yields much higher sensitivity in matrix-assisted laser desorptiony ionization mass spectrometry than does the underivatized oligosaccharide. Digestion of the glycopeptide by a exogly...
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Spectra-structure relationships were investigated for estimating the anomeric configuration, residues and type of linkages of linear and branched trisaccharides using C-NMR chemical shifts. For this study, 119 pyranosyl trisaccharides were used that are trimers of the or anomers of D-glucose, D-galactose, D-mannose, L-fucose or L-rhamnose residues bonded through or glycosidic linkages o...
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We obtained the nearly complete structural elucidation of oligosaccharide components, including sequence, linkage, and even stereochemistry in the picomolar levels. The "catalog-library" approach is used for elucidating the structures of minor components in a mixture of oligosaccharides. Oligosaccharides released from a family of glycoproteins are often composed of a small finite set of monosac...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1997
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.94.5.1629